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Attogene specializes in custom ELISA development for clients who need validated immunoassays that perform reliably in their specific matrix, sensitivity range, and regulatory environment. Whether you require a sandwich ELISA, competitive ELISA, or multiplex panel, our scientists engineer each assay from the ground up — selecting antibody pairs, optimizing blocking conditions, and validating performance to ensure you get data you can trust.

Our custom ELISA development services support a wide range of applications, including pharmaceutical drug development, food and environmental safety testing, aquaculture health monitoring, and academic research. Browse our ELISA product catalog to see validated assays already available off the shelf, or explore our full Attogene product list spanning lateral flow, qPCR, enzyme, and nucleic acid platforms. For project inquiries, visit our Services Division page.

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We engineer the right format for your target, matrix, and throughput requirements. Attogene has hands-on experience developing all major ELISA configurations, and can recommend the optimal format based on your analyte’s molecular weight, available antibodies, and detection sensitivity goals.

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Every custom ELISA development engagement begins with a discovery call to define your target analyte, sample matrix, intended use, and regulatory requirements. From there, Attogene’s scientists take ownership of the full development pipeline:

• Antigen & Antibody Sourcing: We identify and procure recombinant antigens and candidate antibody pairs, or work with your proprietary reagents.
• Antibody Pair Screening: Systematic checkerboard titrations identify capture/detection pairs that deliver the highest signal-to-noise ratio.
• Assay Optimization: Buffer formulations, blocking agents, incubation conditions, and plate coatings are systematically optimized for your matrix. We also offer pre-coated plates including our Streptavidin Coated ELISA Plates (EL2055), Goat Anti Rabbit Coated Plates (EL2058), and Mouse Anti FAM/FITC Coated Plates (EL2059) to accelerate your development.
• Validation: Sensitivity, specificity, accuracy, precision (intra- and inter-assay CV), linearity, and spike-recovery are characterized per your regulatory guidance (FDA, EPA, ICH Q2).
• Kit Formulation & Transfer: Validated reagents are formatted into a ready-to-use kit with a full SOP. Need a self-build starting point? See our ELISA Do-it-Yourself Development Starter Kit (EL2061).

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While many clients come to us for fully custom ELISA development, Attogene also maintains a growing catalog of validated, off-the-shelf ELISA kits that may meet your needs immediately. If your target is already covered, you can order directly and start testing today.

View the complete Attogene ELISA product page or the full Attogene product list for all available kits, pre-coated plates, and assay accessories.

Attogene was founded by scientists who have developed immunoassays for some of the most demanding applications in environmental monitoring, aquaculture, and pharmaceutical research. That deep bench experience means we anticipate problems before they slow your project — and we bring solutions, not just data.

Every custom ELISA development project includes milestone reporting and open communication with your team throughout. You always know where your assay stands and what comes next. Explore our full product catalog, browse the ELISA product line, or meet the team behind the science. You may also be interested in our lateral flow assay development services and nucleic acid detection platforms as complementary detection strategies.