FAM and Biotin End-labeled 23S Amplicon fragment of 874 base pairs Derived from a strain of Microcystis aeruginosa. Amplification was performed using a set of specific primers:
Forward primer: 5′- /5Biosg/AGGGGTAAAGCACTGTTTCG -3′ 20ul of a 10uM solution
Reverse primer: 5′- /56-FAM/CCTTCTCCCGAAGTTACG -3′ 20ul of a 10uM solution
This PCR Amplicon is functionally tested to work with the Universal Nucleic Acid Lateral Flow ImmunoAssay (NALFIA) kit catalog number: AU2034 -01 and a perfect control to be used for a larger nucleic acid tagged amplified product with the Universal Lateral Flow systems.
Sequenced using mini ion with Microcystis aeruginosa UTEX #2388:
Results:
99.76% match: Microcystis aeruginosa NIES-933 gene for 23S rRNA, partial sequence
99.51% match: Microcystis aeruginosa UTEX LB 2388 23S ribosomal RNA gene, partial sequence
Sequence identified using Nanopore from gel purified PCR product produced using primer set used in attogene kit:
ATTTTTTTGTCCTCTACTGGTTCGGTGCTATGTAAAACGGGTGTTTAACCGTTTTCGCATTTATCGTGAAACGCTTTCGCGTTTTTCGT
GCGCCGCTTCAAGCACTGTTTCGTAACTTCGGGAGAAGGAGTAGGGGTAAAGCACTGTTTCGCCGCGGGCTGGGAGACCGGTACCAA
GGCGAGGCAAACTCTGAATACGGCTAAAAAAACTACCAGTGAGACGGTGGGGGATAAGCTTCATCGTCAAGAGGGAAACAGCCCAGA
CCACCAGCTAAGGTCCCCAAATGATAACTAAGTGAGAAAGGAGGTGGGAGTGCATTGACAACCAGGAGGTTTGCCTAGAAGCAGCAA
CCCTTAAAAGAGTGCGTAATAGCTCACTGGTCAAGCGCTCCTGCGCCGAAAATGAACGGGGCTAAGTTATCTACCGAAGCTGTGGACT
ACATTGTAGTGGTAGGGGAGCGTTCTATAGGGGGTGAAGCAGTAGCGGCAAGCAGCTGTGGACTCTATAGAAGTGAGAATGTCGGCT
TAAGTAGCGAAAATGTGGGTGAGAATCCCACACCCCGAAACCCCAAGGTTTCCTCCCGCAAGGCTCGTCCGCGGAGGGTCAGTCAGGA
CCTAAGGCGAGGCTGAAAAGCGTAGTCGATGGACAACGGGTTAACATTCCCGTACCGATTAATGATTGTGCCGGAGAACGGAGAAGGT
CAACGTCAGCTGGATGTTGGTTACCAGTGCAAGCAGTCGAGGCGATGAGAGGTGGCGAAAACACCAGGAGCTAAGACGCGAGTCCCAC
CTCCCACGGGAGGGAAGTGGCGTTGATCAAGCTTCCTAGAAAAGCCCGAACCACGTTAATCATTAATTGCCTGTACCCGAAACCGACAC
AGGTGGGGAAGTAGAGAATACTAAGGGGCGCGAGATAACTCTCTCTAAGGAACTCGGCAAAATGACCCCGTAACTG
Perfect as a control for Attogene NALFA
