Colloidal Gold for Lateral Flow (60nm) is Attogene’s 60nm colloidal gold (catalog AU2015), a highly stable and uniform suspension of 60 nm gold nanoparticles for making lateral flow conjugates. It is the largest particle in Attogene’s colloidal gold line, with a diameter of 60 nm ± 4.5 nm, and can be supplied from 1 OD to 100 OD.
The gold is functionally tested in lateral flow and made by Attogene in Austin, Texas, in a facility designed for rapid turnaround and batch-to-batch consistency. Bulk pricing and manufacturing supply contracts are available.
Key Features
- Largest standard size: 60 nm ± 4.5 nm, completing the 10, 30, 40 and 60 nm range.
- Uniform shape: more than 95% spherical particles with a size CV below 12%, per Attogene’s colloidal gold literature.
- Low batch-to-batch variation: ±2 nm, so optimized conditions transfer to new lots.
- Gentler pelleting: Attogene’s guide lists the lowest centrifugation range of its sizes for 60 nm conjugates.
- Functionally tested in a lateral flow format.
- Made in Austin, TX, with bulk pricing and supply contracts.
Specifications
Each 60nm colloidal gold order is supplied at 1 OD as standard. Specifications for 1 OD 60 nm gold:
- Particle diameter: 60 nm ± 4.5 nm
- Particles per mL: 1.5–2 × 10¹⁰
- Gold concentration: 3.6–4.1 × 10⁻² mg/mL
- Molar concentration: 2.5–3.4 × 10⁻¹¹ mol/L
- Concentration options: 1 OD standard; 1–100 OD on request
- Pack sizes: 30 mL, 100 mL, 500 mL and 1 L
- Handling: do not freeze; freezing irreversibly aggregates colloidal gold.
For larger quantities, a custom OD or a lot-specific data sheet, contact sales@attogene.com.
Who It’s For
- Lateral flow developers screening particle size who want the largest Attogene gold to compare with 10nm, 30nm and 40nm Colloidal Gold.
- Researchers making their own conjugates by passive adsorption of antibodies or other proteins.
- Manufacturers who need consistent material and supply agreements.
How to Use 60nm Colloidal Gold in Your Assay
Attogene’s Gold Conjugation Technical Guide covers the full workflow. A condensed version:
- Prepare buffers and antibody first. Adsorption is complete within seconds of mixing. Desalt the antibody into 10 mM HEPES or another clean buffer; BSA, Tris, glycine, glycerol and azide interfere with conjugation.
- Screen pH in a 96-well plate: 100 µL of 1 OD gold, 10 µL of 22 mM buffer per pH (5.5 to 10) and 10 µL of antibody at 100 µg/mL, then 25 µL of 1.5 M NaCl after 15 minutes. Read after 5–10 minutes; red wells are stable.
- Titrate the antibody at the best pH to find the minimum stabilizing amount, then add a 10–20% excess to allow for handling losses.
- Scale up and block. Add the antibody to the gold while vortexing gently, incubate, then block with BSA to 1% final.
- Pellet the conjugate. The guide lists ~700–4,000 × g for 15–20 minutes for 60 nm particles. Start at the low end for a soft pellet that resuspends easily, then resuspend in conjugate diluent.
- Check quality and function with a UV-Vis spectrum, a salt challenge and a dot-spot test on blank strips.
Download the Gold Conjugation Technical Guide and Colloidal Gold Flyer (PDF).
How Particle Size Fits Into Optimization
Size is one of the core variables in a gold-based lateral flow test, together with gold concentration, antibody loading and the reader. Attogene’s article The Impact of Colloidal Gold on Lateral Flow Optimization showed that changing particle size changed how a competitive assay read by eye, so it is worth testing more than one size with your antibody pair.
At 1 OD, the 60 nm product contains 1.5–2 × 10¹⁰ particles/mL, the fewest particles per OD of Attogene’s four sizes. Because conditions are specific to each particle, repeat the pH screen and antibody titration when you change sizes.
Also consider Protein A Colloidal Gold or Streptavidin Colloidal Gold for ready-made conjugates, and the Universal Lateral Flow Assay Kit (Particles Not Included), a nucleic acid dipstick kit that leaves the choice of detection particle to you.
Frequently Asked Questions
What OD is the 60nm colloidal gold?
Standard products are 1 OD. Attogene can supply 60nm colloidal gold from 1 OD to 100 OD; contact sales for pricing.
What centrifuge speed should I use for 60 nm conjugates?
Attogene’s guide lists roughly 700–4,000 × g for 15–20 minutes. Use the lowest speed that gives a nearly clear supernatant and a soft pellet.
Is a spec sheet available?
No separate spec sheet is posted for the 60 nm size; the key specifications are listed above. For lot-specific data, email sales@attogene.com.
Can I buy more than 1 L?
Yes. Bulk pricing and manufacturing supply contracts are available.
Does Attogene offer conjugation services?
Yes. Attogene’s lateral flow services include colloidal gold conjugation, pad and buffer optimization, prototype manufacturing and scale-up.
For research use only. Not for use in diagnostic procedures. Questions? Call 512-333-1330 or email sales@attogene.com.
For larger sizes contact us: sales@attogene.com
