The Aflatoxin B1 Lateral Flow Kit (AU2051) is an aflatoxin B1 rapid test for screening ground grain samples at or above 2 ppb. A simple buffer extraction is followed by a 15-minute cassette test, and results can be read by eye or with a lateral flow reader. Each kit contains 10 cassettes and everything needed to extract and run 10 samples.
Aflatoxins are toxic metabolites produced by certain Aspergillus molds, and aflatoxin B1 is the most common and most potent of them. Contamination can occur in the field or during storage, so routine screening of grain and feed is a core part of food and feed safety programs.
Key Features
- 2 ppb screening level: Screens grain samples for aflatoxin B1 at or above 2 ppb.
- 15-minute run time: Fast results after a short extraction.
- Simple extraction: 1 g of ground sample, 2 mL of buffer, 3 minutes of shaking.
- Visual or reader readout: Read by eye, or use a lateral flow reader for numerical results.
- Semi-quantitative option: Run known aflatoxin B1 standards alongside samples to estimate levels from 0 to 20 ppb.
- Room-temperature storage: No refrigeration needed.
What’s in the Kit
Each aflatoxin B1 rapid test kit includes:
- 10 aflatoxin B1 test cassettes, individually foil-pouched with desiccant
- 50 mL sample extraction/running buffer
- 10 sample extraction tubes
- 10 fixed volume pipettes, 200 µL
You will also need: a timer and a marker for labeling. Aflatoxin B1 control solutions are not supplied and are needed only for semi-quantitative testing.
Who It’s For
- Grain elevators, mills and feed producers screening incoming lots.
- Food and feed QA labs that need a quick screen before confirmatory testing.
- Researchers studying mycotoxin contamination in crops and stored grain.
How to Use the Aflatoxin B1 Rapid Test
Extraction
- Collect the sample and grind it.
- Add 1 g of ground sample to an extraction tube.
- Add 2 mL of sample extraction/running buffer.
- Vortex or shake for 3 minutes, then let the solution settle for 2 minutes.
Running the test
- Wearing gloves, remove cassettes from their foil pouches and label them.
- Negative control first: transfer 200 µL of extraction/running buffer into the sample port of a cassette and start a 15-minute timer.
- Sample: transfer 200 µL of the settled extract into the sample port of a new cassette and start a 15-minute timer.
- Read the cassettes by eye or in a lateral flow reader.
Full instructions are in the AU2051 manual (PDF).
Reading the Results
This is a competitive assay, so more aflatoxin B1 in the sample produces a lighter test line. Compare each sample cassette with the negative control cassette run in parallel. A visible control line is required for a valid result.
- Test line darker than or equal to the negative control test line: below the detection limit.
- Test line lighter than the negative control: 2 ppb or greater.
- No test line, control line only: 20 ppb or greater.
Read results within 15 minutes after the run is complete, since line intensities can change as strips dry. Do not use previously run, dried strips for semi-quantitative comparisons.
Confirming Results
The lateral flow kit provides preliminary, qualitative screening results. Positive samples can be confirmed by ELISA, HPLC or other conventional methods. For quantitative lab testing, see our Aflatoxin B1 ELISA Kit.
Frequently Asked Questions
What is the detection level of the aflatoxin B1 rapid test?
The kit screens for aflatoxin B1 at or above 2 ppb in grain samples. A sample with no visible test line indicates 20 ppb or greater.
How long does the test take?
About 5 minutes for extraction and 15 minutes for the cassette run.
Is the result quantitative?
Visual reading is qualitative. You can obtain semi-quantitative results from 0 to 20 ppb by running known aflatoxin B1 standards alongside samples, or numerical readings with a lateral flow reader.
Should I use the rapid test or the ELISA?
Use the aflatoxin B1 rapid test for fast screening of individual samples. Use the ELISA when you need quantitative results for many samples or to confirm positives.
How should the kit be stored?
Store at room temperature and keep cassettes sealed in their pouches until use.
Attogene also offers other rapid food tests, including the SEB lateral flow detection kit.
For research use only. Not for use in diagnostic procedures. Questions? Call 512-333-1330 or email sales@attogene.com.
