Goat Anti-Mouse Colloidal Gold for Lateral Flow (AU2041) is a goat anti-mouse colloidal gold conjugate supplied as 1 mL at 10 OD on 40 nm gold particles. It gives assay developers a ready-made gold label for mouse antibodies, functionally tested on a lateral flow test and suited to both lateral flow development and use in final products.
Mouse monoclonal antibodies are widely used in lateral flow, so a goat anti-mouse (GAM) gold conjugate is a practical tool for screening antibodies or adding a control line. The conjugate is made in a state-of-the-art manufacturing facility that allows rapid turnaround while ensuring batch-to-batch consistency and reliability.
Key Features
- Labels mouse antibodies: one conjugate works with many different mouse primary antibodies, so you do not need a new gold conjugate for each antibody you screen.
- 40 nm gold, 10 OD: a common particle size for visible red lines, supplied concentrated in a 1 mL volume.
- Functionally tested: the GAM gold is checked on a lateral flow test.
- Ready to use: no antibody conjugation chemistry required in your lab.
- Scales with you: suitable for prototyping and for use in a final product, with other ODs and larger amounts on request.
Specifications
Each goat anti-mouse colloidal gold order includes:
- Conjugate: goat anti-mouse (GAM) antibody conjugated to colloidal gold
- Gold particle size: 40 nm
- Optical density: 10 OD
- Volume: 1 mL
- SKU: AU2041
The product listing also shows 10 mL and 100 mL of 10 OD. For a different OD or amount, or for the current lot’s specification details, email sales@attogene.com.
Who It’s For
- Developers screening mouse monoclonal antibodies for a new strip test
- Labs that need a control line chemistry based on mouse IgG and an anti-mouse gold label
- Researchers running feasibility studies on dipsticks before investing in direct conjugates
- Production teams that want a consistent conjugate for final product builds
How to Use Goat Anti-Mouse Colloidal Gold in Your Assay
The steps below are general guidance for working with a secondary antibody gold label. Volumes, buffer composition and pad treatment are assay specific and should be optimized on your own strips.
- Define the job of the conjugate. Use it to label a mouse primary antibody bound at the test line, or as a procedural control captured by mouse IgG at the control line.
- Mix the vial. Gold particles can settle during storage; mix until the dispersion looks uniform before pipetting.
- Start on dipsticks. Add the conjugate to running buffer with your sample and mouse antibody for fast feasibility checks.
- Optimize the ratio. Titrate gold against antibody to balance line intensity and background.
- Move to conjugate pads. Once the chemistry works, apply the conjugate to a conjugate pad for a finished strip. Contact Attogene for pad spraying or spotting recommendations.
Reading Results and Assay Design
Gold conjugates produce a red line that can be read visually or measured with a lateral flow reader. Because a goat anti-mouse label binds any mouse IgG on the membrane, pair it with a capture reagent that is not a mouse antibody, such as a rabbit or other non-mouse capture antibody, or reserve it for the control line.
For rabbit primaries, use Goat Anti-Rabbit Colloidal Gold. Tag-based formats can use Streptavidin Colloidal Gold or Anti-FITC/FAM Colloidal Gold, and Protein A Colloidal Gold binds the Fc region of many IgGs. For pre-made strips, see the Universal Lateral Flow Assay Kit (anti-FAM/FITC-Gold).
Frequently Asked Questions
What is in each vial of goat anti-mouse colloidal gold?
Each 1 mL vial contains goat anti-mouse antibody conjugated to 40 nm colloidal gold at 10 OD.
Can I order a different OD or volume?
Yes. The listing shows 10 mL and 100 mL of 10 OD, and other ODs or amounts are available on request from sales@attogene.com.
Is this goat anti-mouse colloidal gold suitable for final products?
Yes. Attogene offers it for lateral flow development and for use in final products, and larger quantities can be quoted for production.
Is the conjugate functionally tested?
Yes. The GAM gold is functionally tested on a lateral flow test. Ask sales for the current lot’s test details.
Can Attogene develop a complete assay?
Yes. Attogene’s lateral flow development services support projects from feasibility studies and antibody screening through prototype development and scale-up.
For research use only. Not for use in diagnostic procedures. Questions? Call 512-333-1330 or email sales@attogene.com.
