Goat Anti-Rabbit Colloidal Gold for Lateral Flow (AU2040) is a goat anti-rabbit colloidal gold conjugate made with 40 nm gold particles and supplied as 1 mL at 10 OD. The goat anti-rabbit (GAR) antibody conjugate is functionally tested on a lateral flow test and is intended both for lateral flow assay development and for use in final products.
Attogene produces its conjugates in a state-of-the-art manufacturing facility that allows rapid turnaround times while ensuring batch-to-batch consistency and reliability. If your assay needs a different optical density or a larger amount, Attogene can supply it on request.
Key Features
- 40 nm gold particles: a widely used particle size for visible red test and control lines in lateral flow.
- 10 OD, 1 mL: supplied as a concentrated, ready-made conjugate, so there is no antibody conjugation step in your workflow.
- Functionally tested: the GAR gold is checked on a lateral flow test.
- Development to production: suitable for early prototyping and for use in a final product.
- Consistent supply: controlled manufacturing supports batch-to-batch consistency and rapid turnaround.
- Flexible quantities: other OD values and larger volumes are available on request.
Specifications
Each goat anti-rabbit colloidal gold order includes:
- Conjugate: goat anti-rabbit (GAR) antibody conjugated to colloidal gold
- Particle size: 40 nm
- Optical density: 10 OD
- Volume: 1 mL
- SKU: AU2040
The product listing also shows 10 mL and 100 mL of 10 OD. For these or any other OD or volume, email sales@attogene.com for a quote and the current lot’s specification details.
Who It’s For
- Assay developers who use rabbit primary antibodies and want a ready-made gold label for them
- Teams building a control line into a lateral flow strip
- Labs comparing labeling strategies before committing to a direct antibody–gold conjugate
- Manufacturers who need a consistent conjugate for scale-up and final product builds
How to Use Goat Anti-Rabbit Colloidal Gold in Your Assay
A secondary antibody gold conjugate can fit into a lateral flow design in two common ways. The outline below is general guidance; exact volumes, buffers and pad treatments depend on your strip and should be optimized for your assay.
- Choose the role of the conjugate. Use it as the label for a rabbit primary antibody (indirect detection), or as a control reagent that is captured by rabbit IgG sprayed at the control line.
- Resuspend before use. Gold particles can settle over time, so mix the vial until the dispersion is uniform.
- Pick a format. For quick feasibility work, add the conjugate to the running buffer and run dipsticks; for a finished strip, apply it to the conjugate pad.
- Titrate. Test several ratios of gold to rabbit antibody to find the strongest specific line with a clean background.
- Read the lines. Gold produces a visible red line that can be read by eye or with a lateral flow reader.
For recommendations on spraying or spotting conjugate pads, contact Attogene at sales@attogene.com.
How Secondary Antibody Gold Works
Goat anti-rabbit antibodies recognize rabbit antibodies. When a rabbit primary antibody binds its target at the test line, the goat anti-rabbit colloidal gold binds that rabbit antibody and the gold particles accumulate into a visible line. Because one GAR conjugate can label many different rabbit primaries, it lets you screen antibodies or targets without making a new gold conjugate each time.
For mouse primary antibodies, use Goat Anti-Mouse Colloidal Gold. For biotin- or FAM/FITC-tagged formats, see Streptavidin Colloidal Gold and Anti-FITC/FAM Colloidal Gold, or start with pre-made strips in the Universal Lateral Flow Assay Kit (Strep-Gold). Protein A Colloidal Gold is another option for binding antibodies.
Frequently Asked Questions
What particle size and concentration is this conjugate?
It uses 40 nm gold particles and is supplied as 1 mL at 10 OD. Other ODs and amounts are available on request.
Can I use goat anti-rabbit colloidal gold in a final product?
Yes. Attogene offers this goat anti-rabbit colloidal gold for both lateral flow development and use in final products, and larger quantities can be quoted for production.
Is the conjugate functionally tested?
Yes. The GAR gold is functionally tested on a lateral flow test. Ask sales@attogene.com for the current lot’s test details.
Where can I get larger volumes?
The listing shows 10 mL and 100 mL of 10 OD. Email sales@attogene.com for pricing and lead time.
Can Attogene build the assay for me?
Yes. Attogene’s lateral flow development services include antibody pair screening, colloidal gold nanoparticle conjugation, conjugate pad optimization, prototype strip manufacturing and assay sensitivity optimization.
For research use only. Not for use in diagnostic procedures. Questions? Call 512-333-1330 or email sales@attogene.com.
