Attogene’s Universal Lateral Flow Assay Kit (Strep-Gold, AU2034) is a ready-to-use universal lateral flow assay kit for building a rapid dipstick readout for dual-labeled DNA and RNA, including amplification products from PCR, LAMP and RPA, as well as CRISPR (Cas12a) workflows. Each 50-test kit contains pre-striped 4.5 mm dipsticks with 40 nm streptavidin colloidal gold, so you can test your labeled product on day one.
There is no need to stripe capture antibodies or buy striping equipment. Results are ready in 15 minutes and can be read by eye, by photograph or with a lateral flow reader. It is a fast, low-cost way to prove feasibility before investing in custom lateral flow development.
Key Features
- Pre-striped strips: anti-FITC/FAM, anti-DIG and anti-DNP test lines, depending on format, plus a biotin-BSA control line.
- Multiplex up to 3 targets: the 4-line format detects three dual-labeled targets on one strip.
- 15-minute results: qualitative or semi-quantitative readout, visually or with a reader.
- Flexible chemistry: works with any nucleic acid carrying biotin plus FITC/FAM, DIG or DNP.
- Simple storage: dipsticks and running buffer at room temperature; control nucleic acids at 2–8 °C.
- Three formats: AU2034-01 (2-line, anti-FITC/FAM), AU2034-02 (3-line, adds anti-DIG) and AU2034-03 (4-line, adds anti-DNP). Lines are spaced 4.5 mm apart.
What’s in the Kit
Each universal lateral flow assay kit includes:
- 50 lateral flow dipsticks (4.5 mm × 60 mm) in a desiccated canister
- 12 mL RNase-free nucleic acid lateral flow running buffer
- 20 µL positive control nucleic acid per capture label: biotin + FAM in all kits, plus biotin + DIG (3- and 4-line kits) and biotin + DNP (4-line kit)
- One 96-well plate
- Manual
You will also need: a timer, a pipettor and tips, a centrifuge for clearing samples, tubes or microtiter plates, and your molecule of interest labeled with biotin and FITC/FAM, DIG or DNP.
Add-ons: extra 2X running buffer, additional control nucleic acids, and cassetted strips (CO2022-02) can be ordered with the kit. Need a different line configuration or bulk strips? Contact sales@attogene.com.
Who It’s For
This kit is designed for researchers and assay developers who want a visual endpoint for nucleic acid amplification without building a strip from scratch. Typical users include molecular biology labs adding a lateral flow readout to isothermal LAMP or RPA assays, teams screening primer designs, and developers evaluating whether a target is a good candidate for a dedicated lateral flow test.
A Lateral Flow Assay Development Kit for Any Labeled Target
Because the test lines capture a label rather than a specific sequence, this universal lateral flow assay works as a general-purpose lateral flow assay development kit. The same strips can read out many different targets, so one lateral flow kit can support primer screening, multiplex design and feasibility studies across projects. When you are ready to move beyond dipsticks, our custom lateral flow development team can carry your assay into cassettes, custom lateral flow test strips and antibody-based lateral flow immunoassay formats.
How to Use the Universal Lateral Flow Assay Kit
- Add 150 µL of running buffer to a well of the 96-well plate.
- Add your sample. Start with 1–5 µL of labeled product and keep the volume below 15 µL.
- Set up controls: 5 µL of control nucleic acid for the positive control, and buffer only for the negative control.
- Mix each well by pipetting up and down several times.
- Insert one dipstick per well, dip end down, with the arrows pointing up.
- Incubate for 15 minutes, then read by eye, by photograph or with a lateral flow reader.
Full instructions, line positions and troubleshooting are in the AU2034 kit manual (PDF).
How It Works and Reading Results
Each dipstick carries streptavidin-conjugated gold, which binds any biotin-labeled molecule. Your product must carry two labels: biotin, which binds the gold, and one capture label (FITC/FAM, DIG or DNP), which binds its test line. Dual-labeled product is captured at the test line and forms a red-purple line. Unbound gold continues to the biotin-BSA control line.
- Test line present: labeled target detected. Intensity increases with the amount of product.
- Control line fading: normal. Test lines capture the gold first, so the control weakens as test lines strengthen.
- Both lines faint at high input: this is the hook effect. Dilute the sample and re-run.
- No lines: the strip did not run. Repeat with a new strip.
Primer design tips: for PCR, put biotin on the 5′ end of one primer and the capture label on the 5′ end of the opposite primer. For LAMP and RPA, place both labels on primers or a probe that end up in the same double-stranded product. For multiplexing, biotin goes on one primer of each target and each target gets its own capture label. Always include a no-template control, because primer-dimers carrying both labels can produce a test line.
Frequently Asked Questions
What labels do I need?
Biotin plus one of FITC/FAM, DIG or DNP on the same product. A molecule with only one of the two labels will not produce a test line.
Can I use it with LAMP or RPA?
Yes. The universal lateral flow assay kit is designed for amplified DNA and RNA from PCR, LAMP and RPA reactions.
Can it detect more than one target?
Yes. The 3-line kit detects 2 targets and the 4-line kit detects 3, all sharing the same gold and control line.
Is it quantitative?
It is qualitative or semi-quantitative. Line intensity and the test-to-control ratio can be measured with a lateral flow reader.
Are there other versions of this kit?
Yes. An anti-FAM gold version is available: see the anti-FAM gold lateral flow kit. For isothermal work, the LAMP lateral flow assay kit is optimized for LAMP products, and a fluorescent version is also available.
Can Attogene build a custom assay from my results?
Yes. Once the kit has shown feasibility, our lateral flow development services team can design a custom strip or cassette for your target.
For research use only. Not for use in diagnostic procedures. Questions? Call 512-333-1330 or email sales@attogene.com.
