Attogene’s Caffeine Rapid Detection Kit screens urine, water and drinks for caffeine in 15 minutes. Add 1–20 µL of sample to a tube of Sample Dilution/Running Buffer, mix, and add 200 µL to a cassette. Read the result by eye or with a lateral flow reader, against a negative control run at the same time.
- Format: 10 cassettes (5 samples, each with its own negative control)
- Run time: 15 minutes
- Cutoff: 10 ppb caffeine in the buffer tube after the sample is added
- Samples: urine, water, coffee, tea, soft drinks, energy drinks and other drinks
- Kit contents:
- 10 caffeine cassettes
- 10 Sample Dilution/Running Buffer tubes (200 µL; one per sample is used as the negative control)
- 10X Sample Dilution Buffer (10 mL), for diluting concentrated samples
- 10 fixed-volume pipettes (200 µL)
- Manual
Adjustable sensitivity: the sample volume sets the concentration in the sample that matches the 10 ppb cutoff.
| Sample added to the 200 µL tube | Cutoff in the sample |
|---|---|
| 20 µL (urine, water) | about 110 ppb |
| 10 µL | about 210 ppb |
| 5 µL | about 410 ppb |
| 1 µL (drinks) | about 2 ppm (2,000 ppb) |
For samples above the range, dilute them first in 1X Sample Dilution Buffer (1 part 10X buffer + 9 parts distilled water), test again, and multiply the result by the dilution factor.
Typical caffeine in soft drinks (12 oz, 355 mL):
- Mountain Dew Zero Sugar: 68 mg (about 190,000 ppb)
- Mountain Dew or Diet Mountain Dew: 54 mg (about 152,000 ppb)
- Diet Coke: 46 mg (about 130,000 ppb)
- Dr Pepper: 41 mg (about 115,000 ppb)
- Coke, Coke Zero or Pepsi: 34–38 mg (about 96,000–107,000 ppb)
- Barq’s Root Beer: 22 mg (about 62,000 ppb); most root beers are caffeine-free
- Decaffeinated coffee: 2–15 mg per 8 oz cup (about 8,400–63,300 ppb)
- Caffeine-free sodas: 0 ppb
For testing drinks in the field without a pipettor, see the Caffeine Test Kit (Field Use), AU2067-01.
