Bacteroides are abundant anaerobic bacteria in the human gut, making them strong indicators of fecal contamination. The HF183 region, a human-specific 16S rRNA gene marker within Bacteroides dorei, is widely used in microbial source tracking (MST) due to its high specificity and prevalence in sewage. HF183 enables sensitive detection of human fecal pollution in environmental waters.
Attogene’s qPCR kit for Bacteroides is designed for the in vitro analysis of the crucial genetic marker HF183. A gene-specific primer mix targeting the HF183 region is provided for amplification and detection using SYBR Green dye on a qPCR instrument. samples are collected and processed to extract purified genomic DNA (gDNA). A reaction mixture is prepared using provided primers, SYBR Green master mix, and extracted gDNA samples as required. The assembled reaction is then loaded onto the qPCR instrument for amplification. The primer mix utilizes Taq polymerase to amplify the gene region of interest, with the SYBR Green dye binding specifically to double-stranded DNA during PCR amplification. This allows for real-time fluorescence detection across a wide range of qPCR platforms.
