Attogene’s Nicotine Test Kit (Field Use) can be used to detect nicotine in liquid samples.
Format: 10 tests
Run Time: 15 Minutes
Can easily detect >5 ppb in liquid samples. Semi-quantitative with an upper range of ~100 ppb. Perfect for evaluation of nicotine levels in water, Zyn pouch extracts, urine, and saliva. Designed to detect nicotine exposure or verify nicotine-containing products. No cross reactivity with cotinine.
Did you know studies have shown that products labeled as “nicotine-free” can still contain measurable levels of nicotine due to contamination or incomplete purification.
Comparative levels of nicotine in products:
Zyn Nicotine Pouches: Typically 3–6 mg per pouch (millions of ppb when extracted).
Cigarettes: ~1–2 mg nicotine absorbed per cigarette.
E-liquids: Commonly 3–50 mg/mL (3,000,000–50,000,000 ppb).
Biological exposure levels:
Urine: Can range from ~10 ppb to >1,000 ppb depending on exposure.
Saliva: Typically ranges from ~5 ppb to several hundred ppb shortly after use.
Nicotine-Free Products: Expected to be 0 ppb, though contamination has been reported.
Legal and industry standards:
In the United States, products labeled as “nicotine-free” are not always subject to strict quantitative limits, but detectable nicotine may indicate contamination or mislabeling.
The FDA regulates nicotine-containing products under the Tobacco Control Act, requiring accurate labeling of nicotine content.
In the European Union, nicotine-containing products such as e-liquids are limited to a maximum of 20 mg/mL.
