Burkholderia pseudomallei is a Gram-negative, motile bacillus that causes melioidosis, a potentially fatal infectious disease. Due to its high environmental persistence, ability to infect via aerosol, and absence of a licensed vaccine, B. pseudomallei is classified as a Category B bioterrorism agent. The S664 region is a genomic locus that has been identified as highly specific to B. pseudomallei. It is notably absent in closely related species such as B. thailandensis and B. mallei, making it a valuable target for molecular diagnostics. The regions low sequence variability and strong species specificity have led to its incorporation into real-time PCR assays.
Burkholderia pseudomallei (B. pseudomallei), a bacterium that can cause melioidosis, can be found in soil and surface water, especially in Southeast Asia and Northern Australia. It’s also been detected in soil and water samples in the U.S. B. pseudomallei is an aerobic, non-spore-forming, non-fermenting, Gram-negative environmental bacterium that’s widely distributed in the rhizosphere, soil, and water.?
Attogene’s qPCR kit for B. pseudomallei is designed for the In vitro analysis of the crucial genetic marker S664. The S664 gene specific primer and probe mix is provided to be detected through the FAM channel on a qPCR machine. A sample is obtained and washed to extract a clean gDNA sample. A reaction mixture is assembled from primers, probe, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest; while the DNA probe mixture is cleaved during amplification to release a FAM fluorophore. The resulting FAM release can be detected on a variety of qPCR platforms
Burkholderia mallei is a non-motile, Gram-negative bacterium that causes glanders, a highly contagious and often fatal disease primarily affecting horses, but also transmissible to humans. Unlike its close relative B. pseudomallei, B. mallei has a smaller, degenerated genome, reflecting its adaptation to a host-dependent lifestyle. The fliP gene, part of the flagellar biosynthesis operon, plays a key role in distinguishing B. mallei from motile relatives. In B. mallei, this gene is typically disrupted or deleted, contributing to the organisms non-motile phenotype. The absence or truncation of fliP serves as a useful molecular marker in PCR-based assays, aiding in the specific identification of B. mallei and differentiation from other Burkholderia species.
Attogene’s qPCR kit for B. mallei is designed for the In vitro analysis of the crucial genetic marker fliP. The fliP gene specific primer and probe mix is provided to be detected through the FAM channel on a qPCR machine. A sample is obtained and washed to extract a clean gDNA sample. A reaction mixture is assembled from primers, probe, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest; while the DNA probe mixture is cleaved during amplification to release a FAM fluorophore. The resulting FAM release can be detected on a variety of qPCR platforms
Recommended DNA isolation technique – inquire at sales@attogene.com for further details:
