Attogene’s Fluorescent Universal Lateral Flow Assay Kits are a convenient ready-to-use kit for quick and cost-effective development of a fluorescent lateral flow dipstick assay for detection of DNA and RNA products. Fluorescent lateral flow assays can be 2-100 fold more sensitive that gold based lateral flow tests. Detectable using a black light such as a black light UV flashlight or fluorescent lateral flow reader.
Detection of nucleic Acid (DNA or RNA) requires the use of a biotin and FAM-labelled primer during amplification:
Test line: anti-FITC/FAM
Control Line: Biotin
Multiplex detection of nucleic Acid (DNA or RNA) requires the use of a biotin, FAM and Dig labelled primers during amplification:
Test Line #1: anti FITC/FAM
Line #2: anti-Dig
Line #3 Biotin.
Inquire about custom configurations: sales@attogene.com.?
For example, this product can be configured with alternative/custom streptavidin fluorescent particles.
Kit Components:
50 -4.5mm Fluorescent Lateral Flow Dipsticks
10 mL Sample assay running buffer
20 uL control Nucleid Acid
Features & Benefits:
Can be used for development of a lateral flow assay for detection of a variety of different molecules such as amplified DNA products from PCR, LAMP and RPA reactions.
No need to stripe capture antibodies
No expensive equipment required (Black Light)
Cost-effective way to screen for further downstream lateral flow assay development.
Formats (excitation 566, 575nm emission) Streptavidin conjugate pad):
