NALF: Nucleic Acid Lateral Flow – Directly detects nucleic acids exploiting capture and labeled reporter oligonucleotide probes. In this kit the capture probe is immobilized to the nitrocellulose using a biotin-streptavidin complex while the detector nucleic acid is detected using a FAM/FITC gold conjugate. Attogene offers custom capture oligonucleotide conjugation services to attached to the BSA carrier. Chemically attaching the capture probe to BSA forms a more stable conjugate that can be sprayed or spotted for multiplex detection of targets.
The “On Strip” Nucleic Acid Hybridization Lateral Flow Starter Kit provides all the needed components required to attach a biotinylated nucleic acid to a nitrocellulose membrane securly and run tests with complimentry hybridization probes.
Useful for optimizing the amount of FAM labeled hybridization probe to obtain a signal in a lateral flow – “on strip” hybridization technique
Specially formulated and optimized “on strip” hybridization running buffer
Control biotinylated capture probe and FAM labeled hybridization probes are included for verification of testing methods
100 blank strips and enough reagents to run 100 “on strip” hybridization tests.
For more advanced on strip hybridization methods and custom capture probe spraying onto nitrocellulose cards please contact for custom service offering at 512-333-1330 or by e-mail at sales@attogene.com.
