Nucleic Acid Amplification Technology, or NAAT, refers to a family of laboratory methods that copy a specific DNA or RNA sequence millions of times over, turning a handful of target molecules into a quantity large enough to detect. Because amplification pushes even trace amounts of genetic material into a detectable range, NAAT methods sit at the core of modern molecular diagnostics, from infectious disease testing to genotyping and food safety screening.

The most widely used NAAT methods include polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), and CRISPR-Cas based detection systems. Each takes a different technical path to the same goal: turning a small number of target sequences into a signal that can be reliably measured or visualized.

Amplification solves the sensitivity problem, but researchers still need a fast, simple way to read the result. Lateral flow assays answer that need. Rather than running amplified samples through a gel or a fluorescence plate reader, a lateral flow strip gives a visual readout in minutes, without specialized lab equipment. This combination, amplify the target, then read it on a strip, has become the backbone of point-of-care and field-deployable molecular testing.

To make that readout possible, amplification primers or probes are typically tagged with small molecules such as biotin, FAM, or DIG. As the amplified sample migrates across the strip, these tags are captured by matching antibodies or streptavidin printed onto the membrane, producing a visible test line.

Attogene’s Universal Lateral Flow kits are built around this tag-capture principle, which is what makes them broadly compatible across LAMP, RPA, and PCR workflows without requiring a custom-designed strip for every target. Kits are available in multiple capture line configurations, including strips with up to four lines for multiplexed detection of DNP, FAM, and DIG tagged amplicons, so a single strip can be adapted to a range of assay designs.

For CRISPR-Cas based detection, which relies on a distinct reporter-cleavage readout rather than simple tag capture, Attogene offers kits purpose-built for that workflow.

All Attogene lateral flow strips are available in both dipstick and cassette formats, with specialized lateral flow running buffers and blood separation pads available upon request for whole blood applications.

Explore how each method pairs with Attogene’s kits:

• LAMP (Loop-Mediated Isothermal Amplification)
• RPA (Recombinase Polymerase Amplification)
• CRISPR-Cas Lateral Flow
• PCR (Polymerase Chain Reaction)

Looking for a configuration not listed here? Attogene also offers custom lateral flow development services to build a strip tailored to your specific assay. Learn more about our custom NAAT lateral flow services.