Loop-mediated isothermal amplification, or LAMP, is a nucleic acid amplification method that runs at a single, constant temperature, typically around 60-65°C, rather than cycling through the repeated heating and cooling steps required by PCR. This is possible because LAMP uses a strand-displacing polymerase along with a set of four to six specially designed primers that recognize six to eight distinct regions of the target sequence, allowing the reaction to amplify DNA without a thermocycler.

Because LAMP requires only a simple heat source, such as a heat block or even a water bath, it has become a popular choice for point-of-care and field-based molecular testing where lab infrastructure is limited.

LAMP reactions can be engineered to produce amplicons carrying affinity tags, most commonly biotin paired with FAM or DIG, by incorporating labeled primers into the reaction. Once amplification is complete, the tagged amplicons can be applied directly to a lateral flow strip for a visual result in minutes, without needing gel electrophoresis or fluorescence instrumentation to confirm a positive result.

This pairing has made LAMP plus lateral flow one of the most common combinations in rapid infectious disease testing, agricultural pathogen screening, and other field-deployable diagnostic applications.

Attogene’s Universal Lateral Flow kits are designed to capture tagged amplicons using a streptavidin and anti-tag antibody format, which makes them broadly compatible with LAMP assays using biotin, FAM, or DIG labeled primers. Because the capture chemistry is tag-based rather than sequence-specific, the same strip format can be adapted across different LAMP targets simply by changing the primer tags used in the reaction.

Kits compatible with LAMP-based detection include:

AU2034 series (01, 02, 03, 04, 05): configurable strips supporting up to four capture lines for DNP, FAM, and DIG tagged targets, useful for multiplexed LAMP assays detecting more than one target on a single strip.

AU2037: Attogene’s fluorescent Universal Lateral Flow kit, offering a projected 10 to 300-fold increase in sensitivity over standard colloidal gold strips. In internal testing, the kit’s streptavidin-conjugated fluorescent particles were detectable by eye under a standard black light at concentrations as low as 3.9 femtomoles, with tagged oligonucleotide targets detectable down to 19 femtomoles

Additional compatible kits: AU2049, AU2053, AU2054, AU2059, AU2061, AU2063, AU2064 (01, 02, 03), AU2065, EL2021_LF, EL2054, EL2055, EL2059, EL2060, and NA2021.

All Attogene lateral flow strips for LAMP detection are available in both dipstick and cassette formats. Specialized lateral flow running buffers and blood separation pads are also available upon request for applications working directly with whole blood samples.

LAMP-based lateral flow assays are widely used for rapid pathogen detection in clinical, veterinary, and agricultural settings, as well as for on-site genotyping and screening applications where results are needed in minutes rather than hours.

Don’t see the exact configuration your assay needs? Attogene offers custom lateral flow development services to build strips tailored to specific LAMP targets. Learn more about our custom NAAT lateral flow services.