PCR Development Services
Custom PCR Development Services
Attogene provides custom PCR development services for biotechnology companies, environmental laboratories, food safety organizations, pharmaceutical CROs, academic researchers, and government agencies. Our scientific team specializes in end-to-end custom PCR assay development, including qPCR primer and probe design, assay optimization, qLAMP amplification development, multiplexing, internal control integration, and full analytical validation workflows.
Whether you need a custom qPCR detection kit for environmental monitoring, a qLAMP assay for rapid field deployment, or a validated multiplex panel for pathogen surveillance, Attogene delivers scalable nucleic acid assay solutions designed to accelerate your detection workflow from concept to validated product.

Custom PCR Assay Development Capabilities
Attogene’s custom PCR development services cover the complete assay lifecycle — from target selection and primer design through analytical validation and technical transfer. Our team applies deep expertise in molecular biology, environmental genomics, and applied microbiology to deliver assays with the sensitivity, specificity, and reproducibility your application demands.
- qPCR Primer & Probe Design
- Probe & SYBR Green Assay Development
- qLAMP Assay Development
- Multiplex PCR Panel Design
- Internal Amplification Control Integration
- Inhibition Testing & Matrix Optimization
- Limit of Detection (LOD) & Limit of Quantification (LOQ) Studies
- Cross-Reactivity & Specificity Testing
- Nucleic Acid Extraction Workflow Development
- Environmental DNA (eDNA) Assay Development
- Assay Stability & Shelf-Life Testing
- In Silico Target Analysis & Primer Screening
- Analytical Validation Documentation
- Technical Transfer & Kit Formatting
PCR Development Applications
Attogene develops custom PCR assays for a broad range of detection targets and market sectors. Our molecular detection expertise spans environmental monitoring, food safety, human health, animal health, agricultural biosecurity, and applied research — delivering assays validated for real-world sample matrices and deployment conditions.
- Environmental Monitoring & Water Quality
- Harmful Algal Bloom & Cyanobacteria Detection
- Pathogen Surveillance (Legionella, Salmonella, E. coli)
- Antimicrobial Resistance Gene Detection
- Food Safety & Authenticity Testing
- Species Identification & eDNA Monitoring
- Biodefense & Select Agent Detection
- Veterinary & Aquaculture Pathogen Testing
- Pharmaceutical QC & Mycoplasma Detection
- Academic & Government Research Programs
Attogene’s nucleic acid product catalog includes off-the-shelf qPCR kits and qLAMP assays for many of these targets. For organizations requiring custom targets, novel matrices, or proprietary kit formatting, our development team delivers fully validated assays ready for in-house deployment or commercial manufacture.
Why Partner with Attogene for Custom PCR Development
- Integrated Molecular Biology Expertise: Our team brings deep experience in primer and probe design, molecular target biology, sample matrix optimization, and environmental genomics — so your assay is built right the first time.
- Full-Service Development, No Gatekeepers: You work directly with our scientists. Skip the sales queue and get immediate answers on target feasibility, assay design tradeoffs, and validation strategy.
- Complementary Platform Experience: Attogene develops across multiple detection platforms — qPCR, qLAMP, lateral flow assays, and custom ELISAs — enabling multi-format strategies when your application demands them.
- Validated for Real-World Matrices: We routinely develop and validate PCR assays in complex environmental, food, and biological matrices — including water, soil, sediment, tissue, plant material, and culture media.
- Prototype to Finished Kit: From a validated assay protocol to a finished, packaged detection kit with internal controls, lyophilized reagents, and technical transfer documentation — Attogene supports the complete journey to a market-ready product.
Related Products & Services
Attogene offers a growing catalog of off-the-shelf nucleic acid products — including qPCR detection kits for environmental pathogens, harmful algal bloom targets, antimicrobial resistance genes, and more. Organizations requiring lateral flow detection can explore our custom lateral flow assay development services, and those needing protein-level detection can leverage our custom ELISA development services. Browse the full Attogene product list or contact our team to discuss multi-platform detection strategies.

