The 16S ribosomal RNA (rRNA) plays a crucial role in bacterial and archaeal ribosomes. This sequence is highly conserved across bacteria and archaea, it contains variable regions useful for species identification by sequencing the amplicon generated from the primers provided.
oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids
Forward primer: 5’- GGG GAA TYT TCC GCA ATG GG -3′
Reverse primer set: 5’- GAC TAC TGG GGT ATC TAA TCC CAT T -3′ and 5’- GAC TAC AGG GGT ATC TAA TCC CTT T -3′
Product size: 450 base pairs. – effective primer set for nanopore sequencing species identification.
Primer mix of 10uM – 150ul
PCR water -1mL