RNase H Lateral Flow Activity Assay Kit
$250.00
In Stock & Ready to Ship
RNase H activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase H activity in enzyme preparations and determination of RNase H unit activity.
Attogene has developed the first of its kind and patent pending technology that uses a novel RNA/DNA substrate tagged on the 5′ and 3′ end with a Biotin that is attached to our streptavidin colloidal gold reporter molecules and a test line tag. In the absence of RNases H, the gold tagged RNA/DNA molecule will flow up the lateral flow strip and bind to the test line using an anti tag antibody (INDICATING that NO RNase H is detected). When RNases H is present or added, however, the RNA strand of the RNA/DNA substrate is cleaved, and the Biotin tagged 5′ and 3′ tags are spatially separated in solution cauing the test line to disapear.
This test can be used to rapidly and efficiently detect ribonucleases H activity and is a perfect tool for monitoring unit activity or residual RNAse H activity.
You may also like…
Plant and Algae RNA Isolation Kit
$424.00This kit is sufficient for 100 RNA isolations based on:
- 200mg fresh plant
- 1ml algae culture
- 50mg dry seeds.
Compatible with automated systems.
Universal Lateral Flow Assay Kit (Strep-Gold)
$125.00 – $185.00- 50 Lateral Flow Dipsticks (4.5mm)
- 10 mL Sample assay running buffer
- 96 well plate
- Controls
Universal Human IgG/IgM Lateral Flow Serology Kit (Gold)
$250.00Antibody tests are a method of choice to determine if a person has been exposed to a pathogen or not. They are also incredibly valuable in the detection of autoantibodies that can be found in human autoimmune disorders. In this test, a biotinylated antigen (User supplied) is mixed with a biotinylated rabbit IgG (bind to goat anti rabbit control line) and sample (human sera or plasma) is simply mixed into with the specially designed assay running buffer in a well of the supplied 96-well plate, mixed and is then added to the sample port of the cassette. Generally, the reaction is complete in 10-15 minutes. It is very important to note that the relative stoichiometry between the biotinylated antigen, biotinylated rabbit IgG added, and the streptavidin gold is critical for assay optimization. The appropriate concentration of biotinylated antigen to use with strips is dependent upon the purity and sequence and a standard curve can be used to determine the relative ratio (generally between 1ng-100ng per test). A positive control line (biotin-rabbit IgG) antibody will bind to the goat anti rabbit (GAR) line on the test to ensure the assay is running appropriately.
RNaseAlarm – Lateral Flow RNase Activity Assay Kit
$250.00RNase activity in a convenient and sensitive colormetric assay that delivers results in real time. Great for Quality Testing for RNase contamination of materials and supplies.
Fluorescent Universal Lateral Flow Assay Kit (Quantum Dots)
$325.00 – $385.00- 50 Lateral Flow Dipsticks
- 10 mL Sample assay running buffer
