Primer design oasig lyophilised 2X qPCR Master Mix is optimised for use in real-time PCR. The product is stable at ambient temperatures for at least 18 months and can be conveniently shipped and stored at room temperature. The master mix contains a thermo-stable TAQ Polymerase as well as buffer, dNTPs, MgCl2 and stabilisers at concentrations optimised for the enzyme. The master mix has been freeze-dried to produce a room temperature stable preparation.
The kit includes the lyophilised master mix, resuspension buffer and a tube of ROX dye which can be added as required when the master mix is to be used on hardware platforms that use ROX as a passive reference dye.
The resuspended solution requires only the addition of your cDNA, primers and probe to be PCR ready lyophilised 2X qPCR Master Mix.