The 16S ribosomal RNA (rRNA) plays a crucial role in bacterial and archaeal ribosomes. Oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria are perfect for use as an internal amplification control with our Microcystin, ATX and AETK and qPCR kits
Forward primer: 5’- AGCCACACTGGGACTGAGACA -3′
Reverse primer set: 5’-TCGCCCATTGCGGAAA -3′
Probe 1: 5’-/5SUN CCT ACG GGA /ZEN/ GGC AGC AGT GGG /3IABkFQ-3′
Product size: 73 base pairs. – effective primer set for .cyanobacteria internal amplification control
Primer mix of 10uM – 150ul
PCR water -1mL