UDGin™ PCR Cleaner Mix is an efficient tool for carry-over contamination prevention in qPCR or PCR. It is optimized for the use in conjunction with highQu qPCR or PCR products.
UDGin™ PCR Cleaner Mix, is a ready to use 20X mix of optimally combined Uracil-DNA Glycosylase (UDG) and dUTP in a buffer with stabilizers. 1 µl of UDGin™ PCR Cleaner Mix added into each 20 µl PCR reaction prevents amplification of DNA carried over from previous PCR performed with dUTP.
During 10 minutes incubation before the PCR start, UDG hydrolyzes the N-glycosilic bond between uracil and sugar leaving apyrimidinic sites in uracil containing DNA which is then cleaved by heat during the first PCR cycle, thus only the newly added template is amplified. UDG is inactivated during the initial PCR denaturation step and does not destroy newly synthesized dUTP containing DNAs. PCR products obtained when UDGin™ PCR Cleaner Mix was used contain uracil, therefore they will be destroyed again by UDG before the next PCR start.
Use of UDG in certain countries for certain applications may be covered by patents and may require a license.