This kit was based on indirect enzyme-linked immune-sorbent assay technology. Recombinant 2019 nCoV spike protein (antigen) was pre-coated onto 96-well plates. The test samples were added to the wells, unbound conjugates were washed away with wash buffer. Then added HRP conjugated anti-human IgM, if there were any anti-nCoV-IgM in the samples, it would form a complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
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