Recombinant SARS CoV-2 spike protein (antigen) is coated onto 96-well plates. The test samples are added to the wells, unbound conjugates are washed. Then HRP conjugated anti-human IgG is added and if there are any anti-SARS CoV-2-IgG in the sample it will form a complex. The TMB substrate is used to visualize the HRP enzymatic reaction to produce a blue color product that changes to yellow after adding stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
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