Rally™ Rapid Ligation Kit is a premium tool designed for fast and highly efficient ligation reactions, cloning or adaptor/linker joining applications. The Kit contains Rally™ T4 DNA Ligase specially formulated to perform faster; and a 2X buffer which includes PEG to accelerate joining of DNA ends.
The combination of both components allows for an efficient and fast ligation reaction of both blunt and cohesive DNA termini eliminating the need of hours or overnight incubations.
Rally™ T4 DNA Ligase, same as the classical enzyme catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of DNA or RNA. It joins both blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.