HighEnd™ Repair Kit is a premium tool designed for rapid and highly efficient DNA end-repair before the ligation reactions. PCR products, sheared or nebulized DNA, restriction-digested DNA, and cDNA can be blunted/phosphorylated in a couple of minutes and are ready for an efficient blunt-end ligation and cloning.
The Kit includes HighEnd™ Repair Blend – an optimized mix of T4 DNA Polymerase and T4 Polynucleotide Kinase. The 5′→3′ polymerase and 3′→5′ exonuclease activities of T4 DNA Polymerase form the blunt-ended DNA. T4 Polynucleotide Kinase phosphorylates 5′ DNA ends. The resulting DNA is a high-quality blunt-ended substrate for T4 DNA Ligase.
Up to 1-5 microgram of the linear DNA can be blunted and phosphorylated in one 20 min reaction. After the thermal inactivation, the reaction mixture can be used for blunt-end ligations.
HighEnd™ Repair Kit is an ideal choice for preparing for ligations the PCR products obtained with high fidelity polymerases like ALLin™ HiFi DNA Polymerase (HLE0201).