Derived from our HiFi polymerase, the highQu ALLin™ Mega HS HiFi DNA Polymerase provides much lower error rate PCR with a 100 higher fidelity compared to Taq. Compared to Mega HiFi, this hot start enzyme version allows for even higher sensitivity and specificity of PCR as well as for a room-temperature reaction setup and is an excellent choice for multiplex reactions. The ALLin™ Mega HS HiFi DNA Polymerase is engineered to be much faster and to generate a higher yield of long PCR products up to 20 kb from complex GC-rich templates. Therefore the ALLin™ Mega HS HiFi DNA Polymerase is an excellent choice for long and very complex PCR applications where the highest fidelity is demanded. It is an enzyme of choice for cloning and all kind of sequencing applications including NGS. Generated blunt-ended PCR products are suitable for ligation into blunt vectors. To increase ligation efficiency, the use of HighEnd™ Repair Kit (HER0101) is recommended.
The convenience of ALLin™ Mega HiFi DNA Polymerase is maximized by the use of 2X Red Mastermix providing the advantage of reduced pipetting and direct gel loading. ALLin™ Mega HiFi Red Mastermix, 2X is premixed with red dye and density reagents for direct loading on the gels after the PCR. In a 2% agarose TAE gel the dye migrates with~350 bp DNA, in 1% agarose TAE gel with ~ 600 bp DNA fragments. The master mix is even supplied with PCR Water, and the only thing to add is the template with primers.