Naegleria fowleri also known as the “brain eating amoeba” is a bacteria-eating amoeba that can potentially be pathogenic. It can cause naegleriasis, a sudden and severe brain infection. Naegleria fowleri is commonly found in warm freshwater and soil and is the only species within the genus of Naegleria that is known to infect humans. Infection does not occur through drinking contaminated water but only if the water goes up into the nasal cavity. The amoeba travels upwards through the cavity to the brain where it destroys the brain tissue. Naegleria fowleri is a thermophilic organism, growing optimally at temperatures up to 115ºF (46.1ºC) and so infections are more prevalent in the summer months. Symptoms of Naegleria fowleri infections maybe be similar to those of bacterial meningitis in its early stages. It cannot be transmitted from one person to another and the symptoms begin to show between 1-9 days after infection. After the onset of the symptoms the disease progresses extremely rapidly and usually causes death within 1-12 days through brain swelling. As a result, fast and accurate detection is critical. Only 3 people out of 138 individuals infected with Naegleria fowleri in the United states from 1962-2015 have been known to survive, putting the fatality rate at over 97%.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
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