The best possible transfection results are obtained by using the original buffers from Eppendorf, which have been tested for sterility as well as for the absence of mycoplasma, endotoxin, and pyrogens, and have undergone a thorough cytotoxicity Test. The hypo-osmolar buffer 36205-60 is for electroplating, and increases transfection efficiency, particularly for mammalian cells. It allows the electrode formation of the cells and, as a result, easier membrane penetration. The sodium-free buffer system is adapted to the inner cell environment and stabilizes the NA+/K+ gradient across the cell membrane, so even highly sophisticated transfection experiments are possible. The iso-osmolar buffer 36205-62 is for electro Fusion of animal cells. The buffer optimizes your cell Fusion parameters by only requiring a few cells in your chamber.